ISOLATION CULTURE AND IDENTIFICATION OF BONE MARROW-DERIVED MESENCHYMAL STEM CELLS IN RATS

[Objective] The aim of this study is to investigate a feasible approach to isolate,purify,culture,amplify and identify bone marrow mesenchymal stem cells(MSCs) of rats.[Methods] MSCs were isolated and purified from bone marrow of rats by density gradient centrifugation and adherent culture methods.Further,CD34、 CD44 and CD105,the surface markers of MSCs,were detected with flow cytometry.[Results] There were small colony of cells grew in 48 hours after culturing and more cells adhered to plastic surface 72 hours after culturing.The amount of adherent cells increased largely and the shape of them became shuttle-shaped or polygon,and more than 70% of the cells were fused on the 7th days.CD44 /CD105 expressed in about 95%,and CD34 expressed in a little of the third primary cultured MSCs.It meant over 90% of the cells were verified to be MSCs.[Conclusion] MSCs can be isolated and cultured successfully by density centrifugation and adherent culture methods.