Monitoring of Ethanol after Drinking Alcohol

Objective To establish a gas chromatography method for detecting ethanol in blood and research metabolic mechanism of ethanol and investigate ethanol level in blood after drinking alcohol that trouble drivers. Methods Sampling blood from finger twig and analyzed by straight inject GC. Statistical analysis of relationship of ethanol concentration level in blood and time-response of drivers after drinking alcohol was made. Results When ethanol concentration were 0.0758～1.561 mg/ml, the relative standard deviation (RSD) was 4.25%～2.89%. The detection limit of blood ethanol was 0.005 mg/ml. Experiment data indicated that there was significant correlation of drinking alcohol amount and blood ethanol concentration (R 2 =0.9732). Investigation founded that blood ethanol concentration more than 89.9 mg/dl for 80% samples of 150 troubled drivers after drinking alcohol.