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2011•Zhongguo yufang shouyi xuebaoRequires access

Identification of monoclonal antibodies against the epitopes on simian immunodeficiency virus p27 capsid protein

Juanjuan Qu

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Abstract

In this study,three monoclonal antibodies(MAb) against the p27 capsid protein of simian immunodeficiency virus(SIV) were developed,designated p27-A5,p27-C7 and p27-D2.The epitopes on p27 to these MAbs were identified with synthesized short peptides using indirect ELISA.The results showed that MAb of p27-A5 and p27-C7 recognized the same epitop of p27,which was located within sites of aa 61 to aa 76(61SEGCTPYDINQMLNCV76).In addition,the MAb p27-D2 reacted with another epitop of p27 that was located within sites of aa 191 to aa 206(61EQTDAAVKNWMTQTLL76).These results would be useful for further study of p27 the antigenic properties and the development of serological detection methods for SIV.

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What this paper is about

In this study,three monoclonal antibodies(MAb) against the p27 capsid protein of simian immunodeficiency virus(SIV) were developed,designated p27-A5,p27-C7 and p27-D2.The epitopes on p27 to these MAbs were identified with synthesized short peptides using indirect ELISA.The results showed that MAb of p27-A5 and p27-C7 recognized the same epitop of p27,which was located within sites of aa 61 to aa 76(61SEGCTPYDINQMLNCV76).In addition,the MAb p27-D2 reacted with another epitop of p27 that was located within sites of aa 191 to aa 206(61EQTDAAVKNWMTQTLL76).These results would be useful for further study of p27 the antigenic properties and the development of serological detection methods for SIV.

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Available abstract

In this study,three monoclonal antibodies(MAb) against the p27 capsid protein of simian immunodeficiency virus(SIV) were developed,designated p27-A5,p27-C7 and p27-D2.The epitopes on p27 to these MAbs were identified with synthesized short peptides using indirect ELISA.The results showed that MAb of p27-A5 and p27-C7 recognized the same epitop of p27,which was located within sites of aa 61 to aa 76(61SEGCTPYDINQMLNCV76).In addition,the MAb p27-D2 reacted with another epitop of p27 that was located within sites of aa 191 to aa 206(61EQTDAAVKNWMTQTLL76).These results would be useful for further study of p27 the antigenic properties and the development of serological detection methods for SIV.

Key concepts: Capsid, Monoclonal antibody, Epitope, Simian immunodeficiency virus, Virology, Biology, Serology, Antigen

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