Analysis of CD44 and CD_(95) Expression on CD_4~+ T Lymphocyte of HIV-1 Infected Subjects after Effective HAART

Objective To explore the changes of CD_(44) and CD_(95) expression on CD4+ T lymphocyte of HIV-1(human immunodeficiency virus-1) infected persons after effective highly active antiretroviral therapy(HAART).Methods Expression of CD_(44) and CD_(95) on CD4+ CD_(45)RA+T and CD4+CD_(45)RO+T subsets were determined by flow cytometer in both HIV infection group and control group.Expression level of CD(44) and CD_(95) was compared between the two groups.And relationship of CD_(44) and CD_(95) was also analyzed.Results The expression levels of CD44 on CD4+CD_(45)RA+T and CD4+CD45RO+T in HIV infection group were 21.17±8.36 and 28.40±10.49 respectively,both of which were statistically lower than the control group (37.65±2.92 and 51.03±4.12 respectively).However,the expression levels of CD_(95) on CD4+ CD_(45)RA+T and CD4+CD_(45)RO+T in HIV infection group(2.54±1.49 and 19.19±6.96 respectively) were both statistically higher than the control group(1.51±0.11 and 13.68±1.37 respectively).When dividing the patients into A,B,C stage according to the stage standard of AIDS by US CDC,the tendency mentioned above was still the same.And the abnormal expression of CD_(44) and CD_(95) was most obvious in the stage B.After effective HAART,abnormality of CD_(44) and CD_(95) expression could be changed to some extent,but not restore to the normal level.It was also found a positive correlation of expression level between CD_(44) and CD_(95).Conclusions Expression level of CD44 cannot predict the relative expression amount of the virus.Homing of renascent CD4+ T lymphocytes accompany with the increased apoptosis of themselves.