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Rice Genetic Map Construction Based on Elite Indica rice,Jiafuzhan

Kefu Zhou

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Abstract

Two recombination inbred lines(RIL) derived from Jianfuzhan×Guanglu′ai 4 hao and Jiafuzhan × Minghui 86(abbr: GJ and MJ86)were constructed.559 pairs of SSR markers were used to analyze the polymorphism between Jianfuzhan and Guanglu′ai 4 hao or Jiafuzhan and Minghui 86.It was shown experimently that there were 201 and 186 pairs of polymorphism markers respectively and their polymorphism ratio are 35.95% and 33.33% respectively.Two rice genetic maps were constructed based on these markers.The GJ map covered the rice genome about 1 015.7 cM with 127 SSR,whose average interval between two markers on the map was about 8 cM.The MJ86 map covered the rice genome about 1 263.6 cM with 131 SSR.The average interval was about 9.6 cM.These maps could be used for researching the genetic rules of Jiafuzhan elite traits and the inner relations among rice appearance traits,and also they could pave the road to breed new variety of high yield and elite traits by molecular marker-assisted breeding.As both the maps are from RIL,they could be used for the genetic rule analysis and QTL mapping of other traits,at any time.

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What this paper is about

Two recombination inbred lines(RIL) derived from Jianfuzhan×Guanglu′ai 4 hao and Jiafuzhan × Minghui 86(abbr: GJ and MJ86)were constructed.559 pairs of SSR markers were used to analyze the polymorphism between Jianfuzhan and Guanglu′ai 4 hao or Jiafuzhan and Minghui 86.It was shown experimently that there were 201 and 186 pairs of polymorphism markers respectively and their polymorphism ratio are 35.95% and 33.33% respectively.Two rice genetic maps were constructed based on these markers.The GJ map covered the rice genome about 1 015.7 cM with 127 SSR,whose average interval between two markers on the map was about 8 cM.The MJ86 map covered the rice genome about 1 263.6 cM with 131 SSR.The average interval was about 9.6 cM.These maps could be used for researching the genetic rules of Jiafuzhan elite traits and the inner relations among rice appearance traits,and also they could pave the road to breed new variety of high yield and elite traits by molecular marker-assisted breeding.As both the maps are from RIL,they could be used for the genetic rule analysis and QTL mapping of other traits,at any time.

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Available abstract

Two recombination inbred lines(RIL) derived from Jianfuzhan×Guanglu′ai 4 hao and Jiafuzhan × Minghui 86(abbr: GJ and MJ86)were constructed.559 pairs of SSR markers were used to analyze the polymorphism between Jianfuzhan and Guanglu′ai 4 hao or Jiafuzhan and Minghui 86.It was shown experimently that there were 201 and 186 pairs of polymorphism markers respectively and their polymorphism ratio are 35.95% and 33.33% respectively.Two rice genetic maps were constructed based on these markers.The GJ map covered the rice genome about 1 015.7 cM with 127 SSR,whose average interval between two markers on the map was about 8 cM.The MJ86 map covered the rice genome about 1 263.6 cM with 131 SSR.The average interval was about 9.6 cM.These maps could be used for researching the genetic rules of Jiafuzhan elite traits and the inner relations among rice appearance traits,and also they could pave the road to breed new variety of high yield and elite traits by molecular marker-assisted breeding.As both the maps are from RIL,they could be used for the genetic rule analysis and QTL mapping of other traits,at any time.

Key concepts: Quantitative trait locus, Biology, Breed, Genetics, Gene mapping, Genome, Inbred strain, Inclusive composite interval mapping

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