Tissue Distribution and Excretion of PEGylation Polypeptide HM-3 in Rats

To study characteristics of tissue distribution and excretion of PEG modified HM-3 after a single intravenous injection in rats and to provide the basis for clinical trials,the ELISA method was used to determine the drug concentration or the main metabolite excretion in biological samples and in urine,excrement and bile at different time points after intravenous injection of PEG modified HM-3(mPEG-SC_(20k)-HM-3).The drug was mainly distributed in liver and kidney and achieved peak content after administration 1 h.The drug contents were(25.75±5.85)μg/g tissue and(25.58±5.79)μg/g in liver and kidney tissue respectively.The recovery of PEG modified HM-3 in urine and excrement excretion during 0～104 hours were 1.5%and 0.02%respectively,while the recovery during 0～44 hours was less than 0.1%in bile excretion.PEG modified HM-3(mPEG-SC_(20k)-HM-3) has a tissue efficient distribution in liver and kidney and excretion mainly in urine.