Expression of NF-κB subunits P50 and c-Rel protein in primary cortical neurons after oxygen glucose deprivation/reoxygenation

Objective:To investigate the expression of nuclear factor-κB(NF-κB)subunits P50 and c-Rel protein in primary cortical neurons of Wistar rats at different time points of oxygen glucose deprivation/reoxygenation(OGD/R).Methods:The neurons dissociated from the cortex of the neonatal rats were primary cultured and were identified by immunocytochemistry.OGD/R model was established.The study was divided into 6 groups according to different processing methods,including normal group,OGD 4 h treated,OGD 4 h/R 2 h treated,OGD 4 h/R 6 h treated,OGD 4 h/R 12 h treated and OGD 4 h/R 24 h treated groups.The expression of NF-κB P50 and c-Rel protein in neurons was examined by immunocytochemistry method and Western blotting.Results:(1)Immunocytochemistry detection targeting neuron specific enolase(NSE)and beta-Ⅲ tubulin confirmed that the cultured cells were neurons.(2)The expression of NF-κB P50 protein was significantly higher in OGD 4 h group than in control group(P0.05);the expression continued to increase in OGD 4 h/R 2 h and OGD 4 h/R 6 h groups,and reached its peak 6 h after reoxygenation(P0.01),then began to decrease,but the expression in OGD 4 h/R 12 h group was significantly different from that of the control group(P0.05);there was no significant difference between OGD 4 h/R 24 h group and control group.(3)The expression of NF-κB c-Rel protein was similar between OGD 4 h group and the control group;the expression increased in OGD 4 h/R 2 h and OGD 4 h/R 12 h groups and reached its peak 12 h after reoxygenation(P0.01),and did not recover to the normal level in OGD 4 h/R 24 h group(P0.05).Conclusion:Oxygen glucose deprivation/reoxygenation can activate NF-κB subunits P50 and c-Rel in the primary cortical neurons in rats in a time-associated manner.