Expression and Identification of WboAGene of Brucella

To express WboA fusion protein in E.coli BL21 and purify and identify the expressed product,the full-length WboA gene was synthesized by PCR and cloned into vector pET-28a(+).The constructed recombinant plasmid pET-WboA was transformed to competent E.coli BL21 expression under induction of IPTG.The expressed protein was purified with Ni-NTA Agaros,identified by Western blot,and the full-length WboA gene was sequenced.Results showed that both restriction analysis and sequencing proved that recombinant plasmid pET-WboA was constructed correctly.The SDS-PAGE and western blot analysis indicated that the recombinant protein induced by IPTG was about 45 ku.The recombinant protein was purified with Ni-NTA Agaros.