IT-ISJ Markers and Its Application to the Genetic Diversity in Barley

IT-ISJ(Intron-targeted intron-exon splice junction),a new molecular marker,the molecular polymorphism of 34 cultivated hulless barley cultivars(lines) from the Qinghai-Tibet Plateau of China,the Europe and the North America,which was reported firstly in this study.The result showed that,20 primer combinations produced a total of 87 clear bands with an average of 4.35 bands per primer pair,and which 81bands(93.10%) were polymorphic.134 alleles,ranged from 2 to 11,were amplified with an average of 6.7 alleles per primer pair.Genetic similarities ranged from 0.437 to 0.931,with an average of 0.743.The average genetic distance(0.257) was lower among accessions and average genetic diversity(0.59),so the result indicated that the genetic basis is narrower among accessions.Based on the results,IT-ISJ marker could be effectively used in researching genetic diversities,cultivar identifications,construction of genetic map and molecular marker-assisted selection breeding.Therefore,it is very essential that the excellent hulless barley genetic resources should be extensively discovered and excavated to widen the genetic basis of hulless barley genetic resources and increase the efficiency in hulless barley breeding.