Screening of Protein Interacting with HEV by Yeast Two-hybrid from Human Leucocyte cDNA Library
Xia Ningshao
Abstract
Xia Ningshao
Abstract
Using yeast two-hybrid system to screen the protein interacting with HEV from human leucocyte cDNA library,four clones interacting with apoptin were identified.One of them was P38IP(aa510-733).Cell co-immunoprecipitation showed that E2 could bind to P38IP in mammalian cells.
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Using yeast two-hybrid system to screen the protein interacting with HEV from human leucocyte cDNA library,four clones interacting with apoptin were identified.One of them was P38IP(aa510-733).Cell co-immunoprecipitation showed that E2 could bind to P38IP in mammalian cells.
Key concepts: Biology, Immunoprecipitation, cDNA library, Complementary DNA, Two-hybrid screening, Yeast, Virology, Molecular biology