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2008•Journal of Tropical MedicineRequires access

Cloning of Human Flt3 Ligand cDNA and Its Expression in CHO Cells

Cao Kai-yuan

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Abstract

Objective To clone the functional fragment of FL gene and express it in CHO cells.Methods RT-PCR was used to amplify the cDNA of the functional fragment of FL from the total RNA of human peripheral blood.After verification by sequencing,the DNA fragment was then cloned into expression plasmid of pcDNA3.0,and then expressed in CHO cells.Results The human FL cDNA was cloned into vector pcDNA 3.0.Conclusion The eukaryotic expression vectors pcDNA3.0-FL was successfully constructed and expressed in CHO cells.Our work lay the foundation for further research of FL.

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What this paper is about

Objective To clone the functional fragment of FL gene and express it in CHO cells.Methods RT-PCR was used to amplify the cDNA of the functional fragment of FL from the total RNA of human peripheral blood.After verification by sequencing,the DNA fragment was then cloned into expression plasmid of pcDNA3.0,and then expressed in CHO cells.Results The human FL cDNA was cloned into vector pcDNA 3.0.Conclusion The eukaryotic expression vectors pcDNA3.0-FL was successfully constructed and expressed in CHO cells.Our work lay the foundation for further research of FL.

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Available abstract

Objective To clone the functional fragment of FL gene and express it in CHO cells.Methods RT-PCR was used to amplify the cDNA of the functional fragment of FL from the total RNA of human peripheral blood.After verification by sequencing,the DNA fragment was then cloned into expression plasmid of pcDNA3.0,and then expressed in CHO cells.Results The human FL cDNA was cloned into vector pcDNA 3.0.Conclusion The eukaryotic expression vectors pcDNA3.0-FL was successfully constructed and expressed in CHO cells.Our work lay the foundation for further research of FL.

Key concepts: Complementary DNA, Cloning (programming), Molecular biology, Chinese hamster ovary cell, clone (Java method), Biology, Plasmid, Molecular cloning

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