Determination of Organochlorine and Pyrethrpid Pesticides in Mulberry Leaf by Gas Chromatography

A gas chromatography method for the determination of pesticides multi-residues in mulberry leaf was established.The organochlorine and pyrethroid pesticides were extracted from mulberry leaf with petroleum acetonitrile.Then the extracts were cleaned up by alumina neutral-florisil column and eluted by mixed solvents of ethyl acetate and hexane(15∶85,V/V).The extracts were separated by HP-5 and DB1701dual capillary column and detected by electron-capture detector.Results show that the recoveries were from 78.6%to 110.3%,from 78.3%to 109.6%and from 80.1%to 109.1% with RSD ranged from 1.1%to 13.1%,at three spiked mixed organochlorine and pyrethroid levels,respectively.The method has good separation ability and repeatability and can be used in determination of organochlorine and pyrethroid pesticide residues in Mulberryleaf products.