[Effects of nao-yi-an granule on the intercellular expression of IL-6 in the experimental intracerebral hemorrhagic brain of rats].

OBJECTIVE: To define the cellular localization of IL-6 and the effects of the traditional Chinese medicine complex nao-yi-an granule (NYA) on the intercellular expression of IL-6 in the experimental intracerebral hemorrhagic (ICH) brain of rats, so as to investigate the efficacy mechanisms of NYA on ICH. METHODS: ICH rat models were established by stereotaxically injecting 0.4 U type VII collagenase into the right globus pallidus (GP). The distribution and content of IL-6 protein expression together with the reaction of glial cells were investigated with the immunohistochemistry method. RESULTS: Microglial activation was detected from 6 h to 4 d after ICH in the hematoma region, and reactive astrocytes were found from 6 h to 7 d after ICH around the hematoma region; there was an up-regulation of IL-6 in the hematoma at 6 h after ICH; it reached its peak at 12 h, and disappeared on 7 d. CONCLUSION: The major sources of IL-6-positive protein were neurons and the activated microglia in the brain of ICH rats. Stabilizing the increasing expression of IL-6 may be one of the neuroprotective mechanisms of NYA.