Glycosylphosphatidylinositol-anchored protein(GPI-AP)deficiency on blood cells of patients with aplastic anemia and paroxysmal nocturnal hemoglobinuria

Objective To investigate the deficiency of glycosylphosphatidylinositol-anchored pro-teins(GPI-AP) in patients with aplastic anemia (AA) and paroxysmal nocturnal hemoglobinuria(PNH). Methods Flow cytometry (FCM), REDQUANT CD55/CD59 and CELLQUANT CD55/C59 kits were used to assess the expression of CD55 and CD59 on peripheral erythrocytes and granulocytes of 47 AA patients and 42 PNH or AA-PNH patients. Results A variant degree of deficiency of GPI-AP expression was found on granulocytes and/or erythrocytes in 16/47 (34.04 % ) AA patients, and the positivity of deficient cells was significantly lower than PNH and AA-PNH( P 0.01) . Conclusion Varying degrees of GPI-AP frequently existed in AA, PNH and AA-PNH.The ratio of deficient cells was implicated in the differentiation of the related diseases and disease transformation.