2013•Science and Technology of Food IndustryRequires access
Research of extraction of total flvonoids from mulberry leaves by enzyme method with Tween-80
Xiao Dao-a
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Abstract
The extraction of total flvonoids from mulberry leaves with Tween-80 synergistic method was researched in this paper.Using the single factor experiment and orthogonal test to decide the method that in the Tween- 80synergistic method extract total flvonoids from mulberry leaves,and also compare it with the extraction without the surfactant.The results showed that in the extraction process about Tween- 80 synergistic method Tween- 80 's mass concentration was 1%,volume of solvent was 125mL,temperature was 50℃,extraction time was 1.5h. And with this method,the extraction yield of toal flavonoids from mulberry leaves was 2.467%,and in the same condition,without the Tween-80,the yield of it was 1.718%.Thus Tween-80 increase the yield of total flavonoids from mulberry leaves significantly.