Toxic Effects of Several Heavy Metals on the Embryos Development of Spinibarbus hollandi

The present paper deals with the toxic effects of Cu~(2+),Zn~(2+),Cd~(2+) in different ion concentration and the joint toxicity of Cu~(2+)-Cd~(2+) and Pb~(2+)-Zn~(2+) on the embryos development of Spinibarbus hollandi by using the semi-static test method,under the condition of water temperature between 24-26℃.The result suggests that the hatching rate of embryos incubated at the heavy metal solutions of different concentration is lower than the control,and the differences is extreme significant(P0.01).Malformation of embryos was caused by the heavy metal element during the embryos development stage.The development speed of embryo was advanced in the experimental groups(Cu~(2+)≥0.10 mg/L,Cu~(2+)-Cd~(2+)≥0.50 mg/L,Pb~(2+)-Zn~(2+)≥2.00 mg/L),but reduced in the rest of experimental groups of various concentration of metal solutions.The toxic effects of the heavy metals on the embryos development of S.hollandi are Cu~(2+)Cd~(2+)Zn~(2+).The safe concentration of Cu~(2+),Cd~(2+),Zn~(2+),Cu~(2+)-Cd~(2+),Pb~(2+)-Zn~(2+) are 0.009 mg/L,0.065 mg/L,0.023 mg/L,0.011 mg/L,0.523 mg/L respectively.The joint toxicity of Cu~(2+) and Cd~(2+) to the S.hollandi is antagonistic.