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Determination of benzoic acid and salicylic acid in Compound Benzoic acid tincture by HPLC

Lamei Qi

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Abstract

OBJECTIVE To determine benzoic acid and salicylic acid in Compound Benzoic acid tincture by RP-HPLC.METHODS The chromatographic conditions were as follows:Kromasil C18 column (250 mm×4.6 mm,5 μm),methanol and 1% glacial acetic acid (47:53) as mobile phase,a flow rate of 0.8 ml·min-1,detection wavelength of 225 nm.RESULTS The linear range of calibration curve of benzoic acid was 24.03-120.12 mg·L-1(r=0.9997).The average recovery was 100.66% with RSD of 0.70%.The linear range of calibration curve of salicylic acid was 12.01-60.02 mg·L-1(r=0.9992).The average recovery was 100.06% with RSD of 0.78%.CONCLUSION The method is simple,rapid and accurate.It can be used for the quality control of Compound Benzoic acid tincture.

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OBJECTIVE To determine benzoic acid and salicylic acid in Compound Benzoic acid tincture by RP-HPLC.METHODS The chromatographic conditions were as follows:Kromasil C18 column (250 mm×4.6 mm,5 μm),methanol and 1% glacial acetic acid (47:53) as mobile phase,a flow rate of 0.8 ml·min-1,detection wavelength of 225 nm.RESULTS The linear range of calibration curve of benzoic acid was 24.03-120.12 mg·L-1(r=0.9997).The average recovery was 100.66% with RSD of 0.70%.The linear range of calibration curve of salicylic acid was 12.01-60.02 mg·L-1(r=0.9992).The average recovery was 100.06% with RSD of 0.78%.CONCLUSION The method is simple,rapid and accurate.It can be used for the quality control of Compound Benzoic acid tincture.

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Available abstract

OBJECTIVE To determine benzoic acid and salicylic acid in Compound Benzoic acid tincture by RP-HPLC.METHODS The chromatographic conditions were as follows:Kromasil C18 column (250 mm×4.6 mm,5 μm),methanol and 1% glacial acetic acid (47:53) as mobile phase,a flow rate of 0.8 ml·min-1,detection wavelength of 225 nm.RESULTS The linear range of calibration curve of benzoic acid was 24.03-120.12 mg·L-1(r=0.9997).The average recovery was 100.66% with RSD of 0.70%.The linear range of calibration curve of salicylic acid was 12.01-60.02 mg·L-1(r=0.9992).The average recovery was 100.06% with RSD of 0.78%.CONCLUSION The method is simple,rapid and accurate.It can be used for the quality control of Compound Benzoic acid tincture.

Key concepts: Benzoic acid, Chemistry, Tincture (heraldry), Calibration curve, Salicylic acid, Chromatography, Acetic acid, High-performance liquid chromatography

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