The effect of Xiaochaihu granule and Chaihu oral liquid on the pharmacokinetics of intragastrically administered cyclosporine A in rats

OBJECTIVE To investigate the possible affect of Xiaochaihu granule and Chaihu oral liquid on the pharmacokinetics of cyclosporine A(CsA) by intragastric administration in rats.METHODS 16 male Sprague-Dawley rats were equally divided into two groups by randomized block design according to weight.Each rat was intragastrically administered CsA(10 mg·kg-1,bid).On the fourth day,after each rat was intragastrically administered CsA(10 mg·kg-1),blood samples(0.6 mL) were collected from the tail vein at 0,1,2,4,6,8,12,24 and 32 h.From day 6 to day 10,each group began to undergo different pretreatments.The Xiaochaihu group were intragastrically co-administered CsA (10 mg·kg-1) and Xiaochaihu granule(2 g·kg-1),At the same time,the rats in Chaihu group were intragastrically co-administered CsA(10 mg·kg-1) and Chaihu oral liquid(2 g·kg-1),then blood samples were collected according to the schedule of the fourth day.The whole blood concentration of CsA was determined by HPLC.Main pharmacokinetic parameters were calculated and compared by statistic analysis.RESULTS Before co-administrated with CsA,no significantly different pharmacokinetic parameters of CsA were found for the two single administration groups.After co-administrated with CsA,AUC0-32h and Cmax were increased significantly(P0.05 and P0.01);t1/2 and V/F were not apparently influenced.There was no significant change in other parameters.CONCLUSION It was indicated that both of the two preparations could apparently increase the intragastric absorption extent of CsA,while they almost had no effect on the drug elimination process.