Study on relationship between erythrocyte indices and genotypes of thalassemia in Guangdong

Objective Toinvestigate the relationship between erythrocyte indices and genotypes of thalassemia in Guangdong. Methods The alpha-thalassemia gene was detected by gap-PCR technology and β-thalassemia gene was detected by RDB technology.RBC,Hb,MCV,MCH,MCHC and RDW were detected by hematology system.150 healthy physical examination cases were used as a control group(NC).The experimental data were analyzed with variance analysis and multiple comparison by SPSS statistical software. Results During January 2011 to December 2013,245 alphathalassemia gene carriers, 208 β-thalassemia gene carriers and 48 αβcompound-thalassemia gene carriers were detected among total 501 cases. With the increase of deleted globin gene numbers, RBC and RDW values were higher than those of the control group, and Hb, MCV, MCH value was lower than those of the control group,which have statistical significance(P0.05).The MCV, MCH and RDW as well as women Hb value of alpha-thalassemia were significantly different from those of beta-thalassaemia(P 0.05).The MCV and MCH values of beta-thalassemia were significantly different from those of αβcompound-thalassemia(P0.05).Among the thalassemia patients, 86.63% were MCV78 fl and MCH27 pg, and 5.39% were MCV≥82 fl and MCH≥27 pg. Conclusions Among the thalassemia patients,RBC was overall increase, while Hb, MCV and MCH had a tendency of decrease, and the heterogeneity of erythrocyte volume was increased. The low level of MCV and MCH may indicate of thalassemia, however it cannot be ruled out thalassemia gene carriers have normal levels of MCV and MCH.