Construction of Stable Shuttle Expression Vectors pQMV and pGMP

The replicon of Pseudomonas plasmid DNA from pUCP19, and a new strong promoter PP303, cloned from Pseudomonas fluorescens P303 strain, were inserted into plasmid pET-29a, a high level expression vector of Escherichia coli. The recombinant shuttle expression plasmid pQMV(4.6 kb) and pGMP(5.6 kb) were obtained respectively. The vector pGMP containing gfp gene was especially available for detection and monitoring. The stability of the two expression vectors were 100%(120 h) in P303 strain of Pseudomonas fluorescens. Both recombinant vectors will be powerful for research on gene expression, regulation and construction of engineered strains of Pseudomonas.