Effect of 2,4-D on callus induction and differentiation of different creeping bentgrass cultivars

The effect of different 2,4-D concentrations and time on callus induction and differentiation of two creeping bentgrass cultivars(Penn A-1 and Penn A-4)were studied.The result indicated that the optimum 2,4-D concentrations for callus induction of Penn A-1and Penn A-4 were 2 mg/L and 4 mg/L,and the callus generation rates were 36.4% and 82.4% respectively.The optimum treatment duration for callus differentiation ranged from 0 to 30 days,and the differentiation rates of Penn A-1and Penn A-4 were 50% and 83%,respectively.Differentiation rate decreased as the increase of 2,4-D treatment duration,and the particle,loose calli that lost differentiation capability were found.