Effect of Zhuyuhuatan Oral Liquid on Cell Apoptosis and Expression of Bcl-2 and Bax Protein Following Intracerebral Hemorrhage in Rats

Objective To probe into the effect of edaravone on the cell apoptosis and the expression of Bcl-2 and Bax protein following intracerebral hemorrhage(ICH) in rats,and explore the protective effect of edaravone on the cerebral injury induced by hemorrhage.Methods Ninety SD rats were randomly divided into 3 groups: Zhuyuhuatan oral liquid group,ICH group and sham group(n=30).The rat model of ICH was established by injection of autologous blood into the caudate nucleus.At different time separately neuronal apoptosis and the expression of Bcl-2 and Bax protein were monitored by Hoechst and immunohistochemistry,respectively.Results Apoptosis and the expression of Bcl-2 and Bax protein were significantly increased in the ICH group as compared with the sham group(P0.01).Treatment of Zhuyuhuatan oral liquid markedly reduced apoptosis ahd the expression of Bax protein in comparison to the ICH group(P0.05 or 0.01),while the expression of Bcl-2 was obviously higher in the Zhuyuhuatan oral liquid group than that in the ICH group(P0.05 or P0.01).Conclusions Zhuyuhuatan oral liquid inhibits the neuronal apoptosis following intracerebral hemorrhage which might be relative to the alleviated oxidative stress,up regulated Bcl-2 protein and down regulated expression of Bax protein.