On Characteristics of Degrading Phenol with Immobilized Strain JY04 by Sodium-aliginate

A phenol degradation bacterium( JY04) which was able to grow on phenol as sole carbon and energy sources was isolated from the activated sludge of the insulating materials factory as the source of bacteria. Orthogonal experiment design was adopted to ascertain the optimal conditions of the immobilization of Strain JY04 and studied the optimal conditions of the immobilized strain to degrade phenol. When SA concentration was 5 %, bacteria embedded ratio 1. 0 : 5. 0 and then treated with 3. 0 % CaCl2for 8 h,the highest degradation rate reached 82. 6 % while the strength of the immobilized cell remained good enough. The efficient biodegradation of phenol occurred when the strain was cultured in the medium( pH 7. 0) containing 0. 4 g / L phenol under 32 ℃ and 25 g inoculation quantum where the highest degradation rate reached 97. 3 % after 42 h. The phenol degradation ability and resistance of the immobilized strain cells against phenol were both higher than the free cells. The immobilized cell is a valid strain that can be used to treat phenol wastewater at high concentration.