The Effect of Early Intervention on NSCs Differentiation of Dentate Gyrus in Brain Damage Rats after HIBD

Objective: To study the impact of early intervention on differentiation of neural stem cells(NSCs) in brain damage rats after HIBD(Hypoxic-ischemic brain injury).Methods: Totally 75 SD(Sprague Dawley) rats aged 7 days were randomly divided into two groups: HIBD model(n = 50) and sham-operated(n = 25).50 HIBD model was produced with Rice method,and randomly divided into HIBD group(n = 25) and early intervention group(n = 25).HIBD group and sham-operated group are normally feed,intervention group are dealed with early intervention,5 rats were killed at 1day,7days,14days,21days and 28days after operation in each group.Each rat was injected with BrdU into its abdominal cavity to mark new cells before killed.Double staining immunofluorescence was used to detect the co-expression of bromodeoxyuridine(BrdU) with neuronal nuclei antigen(NeuN) or glia fibrillary acidic protein(GFAP) in the Dentate gyrus(DG) at different time points.Results:(1) BrdU / NeuN and BrdU / GFAP double staining cells were observed in all 3 groups.(2) In the HIBD and intervention groups,at 14、21 and 28 days after the surgery,the number of BrdU / NeuN and BrdU / GFAP double stained cells increased in the DG compared with the sham-operated group(P 0.01).(3) Obviously more BrdU / NeuN and BrdU / GFAP double-stained cells were found in the early intervention group than that in the HIBD group at 14,21 and 28days(P 0.01).Conclusion: Our results indicate that the brain damae caused by HIBD can induce the differentiation of endogenous NSCs;Early intervention can increase the differentiation of endogenous NSCs.