Synthesis and Characterization of Artificial Antigen for the Organophosphorus Chlorpyrifos

In this paper ,the hapten of the organophosphorus insecticide chlorpyrifos,o,o-diethyl-o-[3,5-dichloro-6-(2-carboxyethyl)thio-2-pyridyl]phosphorothioate was synthesized by using technicalgrade chlorpyrifos reacted with 3-marcapropanoic acid in the hot alkazid solution and its structure was confirmed by IR and GC/MS. The hapten was conjugated to bovine serum albumin (BSA) with the active ester method to form artificial immune antigen. The artificial immune antigen was identified by ultraviolet spectrophotometry and IR spectrum . The results showed that the artificial immune antigen of chlorpyrifos was synthesized successfully. This method made it possible to establish immunoassay of chlorpyrifos.