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[A study on apoptosis induced by As₂O₃ for small cell lung cancer].

Ying Su, Shiren Shen, Peng Gao, Chunping Zou, Z Chen

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Abstract

BACKGROUND: To study apoptosis of small cell lung cancer (SCLC) cell induced by As₂O₃ and enhancement effect of interferon (IFN) on As₂O₃. METHODS: DNA gel electrophoresis, terminal deoxynucleotidyl transferase nick end labeling (TUNEL) and immunocytochemistry were used to detect the apoptosis induced by As₂O₃ with different doses and time and the expression of Bcl-2 or PCNA. RESULTS: Within certain extent of concentration, As₂O₃ could induce apoptosis of SCLC cells. And apoptotic cells increased and expression of Bcl 2 and PCNA decreased with time prolonged or dose increased. The apoptosis inducing effect of As₂O₃ combined with IFN was much higher than that of As₂O₃ alone. CONCLUSIONS: As₂O₃ may induce apoptosis of SCLC cells and the efficiency of apoptosis is related to dose and time. IFNα-2b might enhance the effect of apoptosis on SCLC induced by As₂O₃.

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What this paper is about

BACKGROUND: To study apoptosis of small cell lung cancer (SCLC) cell induced by As₂O₃ and enhancement effect of interferon (IFN) on As₂O₃. METHODS: DNA gel electrophoresis, terminal deoxynucleotidyl transferase nick end labeling (TUNEL) and immunocytochemistry were used to detect the apoptosis induced by As₂O₃ with different doses and time and the expression of Bcl-2 or PCNA. RESULTS: Within certain extent of concentration, As₂O₃ could induce apoptosis of SCLC cells. And apoptotic cells increased and expression of Bcl 2 and PCNA decreased with time prolonged or dose increased. The apoptosis inducing effect of As₂O₃ combined with IFN was much higher than that of As₂O₃ alone. CONCLUSIONS: As₂O₃ may induce apoptosis of SCLC cells and the efficiency of apoptosis is related to dose and time. IFNα-2b might enhance the effect of apoptosis on SCLC induced by As₂O₃.

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Available abstract

BACKGROUND: To study apoptosis of small cell lung cancer (SCLC) cell induced by As₂O₃ and enhancement effect of interferon (IFN) on As₂O₃. METHODS: DNA gel electrophoresis, terminal deoxynucleotidyl transferase nick end labeling (TUNEL) and immunocytochemistry were used to detect the apoptosis induced by As₂O₃ with different doses and time and the expression of Bcl-2 or PCNA. RESULTS: Within certain extent of concentration, As₂O₃ could induce apoptosis of SCLC cells. And apoptotic cells increased and expression of Bcl 2 and PCNA decreased with time prolonged or dose increased. The apoptosis inducing effect of As₂O₃ combined with IFN was much higher than that of As₂O₃ alone. CONCLUSIONS: As₂O₃ may induce apoptosis of SCLC cells and the efficiency of apoptosis is related to dose and time. IFNα-2b might enhance the effect of apoptosis on SCLC induced by As₂O₃.

Key concepts: Apoptosis, TUNEL assay, Terminal deoxynucleotidyl transferase, Proliferating cell nuclear antigen, Immunocytochemistry, Molecular biology, Cell, Cancer research

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