Cloning and analysis of the up-regulated gene of regenerative liver in rats after partial hepatectomy

Objective To clone the differentially expressed genes in liver regeneration for finally elucidating the regulatory mechanism of liver regeneration.Methods In this study,adopting short interval successive partial hepatectomy (SISPH) as experimental model,and recurring to suppression subtractive hybridization (SSH)technique,we constructed 4 h-8 h of 0-4-8-12 h forward subtracted library.Results Thirty-three differentially up-regulated expression ESTs related to liver regeneration were gained,among which 17 ESTs were related to reported genes of liver regeneration,6 up-regulated ESTs were known but firstly reported in liver regeneration and 10 up-regulated ESTs were rat novel genes.Conclusion These results demonstrate that the SSH technology is a powerful tool in analyzing the differentially expressed genes.And the isolation of the differentially expressed genes have laid foundation to further elucidate the molecular mechanism of liver regeneration.