Rapid Detection of the Viability of BCG Vaccine Using Optical Biosensor

Based on the principle of ATP bioluminescence reaction,a rapid method was developed for detecting the viability of BCG(Bacillus Calmette Guerin)vaccine which combined homemade optical biosensor.ATP(adenosine triphosphate)extraction was performed in boiling Tris-EDTA buffer in the experiments.In this study,a high correlation between the viable count of BCG and Relative luminescence intensity(RLU)was obtained with correlation coefficient of 0.9908(P0.01)according to the linearity of ATP standard,and a low Relative Standard Deviation(RSD)of 6.17%.The ATP content of BCG was 10-18 mol/CFU,consistent with the results in papers.The sample volume was 30 μL and the whole test for a sample was completed within 30 min.There was also a high correlation between homemade optical biosensor and the commercial system with correlation coefficient of 0.9676(P0.01).With the characters of easy operation and fast responses,this method can be applied widely in routine quality control to estimate viable count of BCG and other vaccines.