Studies on cloning and expression of the -1, 3-Glucanase and chitinase genes and their potential for fungal resistance

Chitinase and -1, 3-glucanase genes were amplified by PCR procedures and subcloned into an expression vector Pet-28a(+), the prokanyo expression vector of the both genes were constructed and named pETChi and pETGlu, respectively. A SDS-PAGE analysis indicated that chitinase and -1, 3-glucanase genes were expressed highly in E. coli BL21 after induced by IPTG. Challenged with Alternaria alternate showed that chitinase and -1, 3-glucanase genes had good resistance to the pathogenic fungus.