Expression of 11β-hydroxysteroid dehydrogenase 1 in skeletal muscle in rats with type 2 diabetes

Objective To investigate the changes of the expression of corticosteroids metabolic enzymes 11β-hydroxysteroid dehydrogenase 1(11β-HSD1)and glucocorticoid receptor(GR)in skeletal muscle in type 2 diabetic rat model and significance.Methods The Wistar rats were devided into control and type 2diabetic model groups.The models were built with a combination of high fat diet (HFD)and multiple low dose of STZ (30mg·kg-1,twice)injection.8weeks later,the blood of rats were colleted by cutting their tail,the blood glucose was tested with the method of oxidizing enzyme.According to the serologic test and the relevant index of insulin,the damage of islet and the secretion of insulin were determined.The protein expressions of corticosteroids metabolic enzymes 11β-HSD1 and GR in skeletal muscle were examined by Western blotting analysis. Results Compared with control group,the insulin secretion (IS),insulin sensitivity index (ISI)andsecretion index of HOMAβcell(HβCI)were decreased significantly(P0.05),while the insulin resistance index(IRI)was increased significantly(P0.05).The protein expressions of 11β-HSD1and GR in model group were higher than those in control group (P0.05).Conclusion The type 2diabetic model built with a combination of HFD and multiple low dose of STZ injection has the characteristics of high blood glucose,damage of IS function and insulin resistance.In the type 2diabeic models the protein expressions of 11β-HSD1and GR are significantly increased.