2009•Journal of Zhengzhou UniversityRequires access
Apoptosis of cattle lens epithelial cells induced by C2-ceramide
Feng-yan Zhang
Open publisher page 0 citations
Abstract
Aim:To study the effects of C2-ceramide on the apoptosis of cattle lens epithelial cells(LECs), and observe the variation of Caspase-3 activity.Methods:The second generation LECs were treated with different concentrations(25 μmol/L, 50 μmol/L,100 μmol/L) of C2-ceramide for 12 h. After TUNEL staining,the rate of cell apoptosis was calculated; the variation of Caspase-3 activity was determined by Caspase-3 activity assay kit.Results:Cell apoptosis rate and the expression of Caspase-3 activity both increased with the increase of concentrations of C2-ceramide in a dose-dependent manner(P0.05).Conclusion:C2-ceramide could induce apoptosis of LECs,and there is the expression of Caspase-3 activity in this process.