The comparison of the cytotoxicity between cisplatin solution and cisplatin powder in vitro

Objective:To compare the cytotoxicity between cisplatin solution and cisplatin powder in vitro. Methods:Hela, human cell line, was cultured in Dulbecco's modified Eagle's medium (DMEM) containing 10% Neonatal Bovine Serum with or without various concentrations of cisplatin came from solution or powder .Cell viability was examined by methylthiazolyl tetrazolium (MTT) assay and 50% inhibitive concentration(IC50) of cisplatin was examined. Cytotoxicity of cisplatin solution and cisplatin powder in vitro was compared using Student's t-test. Results: Compared with cisplatin powder group, at the same concentration of cisplatin,in cisplatin solution group,cell viability declined and the 50% inhibitory concentration (IC50) of DDP for cisplatin solution was (14.01 ±0.07) μg·ml-1 and IC50 of DDP for cisplatin powder was (15.03±0.26) μg.ml-1(P=0.003). Conclusion: Compared with cisplatin powder,cisplatin solution has a higher cytotoxicity effect in vitro.