2007•Di-san junyi daxue xuebaoRequires access
Expression and characterization of human TALL-1 in Pichia Pastoris
Meng Wang, Yang Yuan, Yang Zhengrong, Guangkuo Li, Xiaohong Liu, Yongping Su, Cheng Tianmin
Open publisher page 0 citations
Abstract
Objective To study the secretion expression of human TALL-1 cDNA in Pichia Pastoris and to obtain recombinant human TALL-1 of good biological activity. Methods The human TALL-1 gene was cloned into yeast expression vector pPIC9K containing AOXI promoter and the sequences of secreting signal peptides. Recombinant plasmid was linearized by Sac Ⅰ and transformed into Pichia Pastoris GS115 competent cells. Positive integrated clones were screened out and cultured in flasks, and hTALL-1 was expressed under the induction of 1% methanol. Biological activities were analyzed with ELISA and MTT assay. Results Human TALL-1 gene was expressed in BMMY after optimization of inducing expression condition. The recombinant could combine with specific antibody and the expressed product could inhibit the proliferation of Hela cells. Conclusion The secretion expression of human TALL-1 was successfully achieved in Pichia Pastoris expression system. The recombinant human TALL-1 could be used for further study on its structure and functions.