A correlation study of tumor necrosis factor polymorphism and MODS

Objective To determine the relation between the susceptibility of MODS and the gene polymorphism of TNF.Methods The single base change of polymorphism in both TNF-α gene and TNF-β gene were analyzed between MODS patients and normal controls by PCR-RFLP.TNF_α and TNF_β plasma concentrations were detected by enzyme-linked immunosorbent assay.Results TNFB2 gene frequency increased significantly in MODS patients compared with normal contrds(67.7% VS 53%,P0.05).Patients with TNFB2 homozygotes had higher TNF_α concentrations as well as mortality(P0.05).No difference of allele frequencies or genotype distribution of TNF_α was found between MODS patients and normal contrds(P0.05).In addition,a significant association was found between the two TNF gene polymorphisms.Haplotype analysis showed that the TNFB2:TNF1 haplotype was significantly risky factor of MODS and TNFB1:TNF1 haplotype was a protective factor of MODS.Conclusion TNFB2 is a susceptive marker for patients with increased TNF α concentration and poor prognosis in MODS.