Apoptosis Induced by Dicycloplatin in Human Ovarian Cell Line A2780

[Purpose] To investigate apoptosis-induced effect of dicycloplatin in human ovarian carcinoma cell line A2780, and to explore its possible mechanism.[Methods] The inhibition of dicycloplatin on A2780 cells proliferation was determined by MTT method.Morphologic change was observed by fluorescence microscopy, Annexin-V-FITC/PI staining multiparameter flow cytometry and TUNEL assay were used to detect apoptotic cells.The activity of caspase-3 and the effect of caspase inhibitor on cell viability were measured.[Results] Dicycloplatin inhibited cell growth with IC50 value was 332.9±26.3μmol/L.The apoptotic events produced by 1×IC50, 2×IC50, 4×IC50 dicycloplatin amounted to 18.5%±5.4%, 39.7%±6.0% and 63.7%±3.4%, respectively, in a dosedependent manner.Cells treated with dicycloplatin exhibited typical morphology of apoptosis.The activity of caspase-3 increased with the increasing apoptosis, and caspase inhibitor z-VAD-fmk partly arrested cell death.[Conclusion] Dicycloplatin can induce apoptosis of A2780 in vitro.Caspase-dependent pathway involves the apoptosis and caspase-independent pathway may be involved as well.