A Research in the Protective Effect of p38MAPK Inhibitor in SAP Rats Model

Objective To investigate the p38MAPK inhibitor SB203580 on the protective effect of severe acute pancreatitis(SAP).Methods 45 rats were randomly divided into three groups:sham operation group(SO,n=15),SAP group(SAP,n=15)and SB203580 group(SB,n=15).The model of SAP was induced by the retrograde injection of 5% sodium taurocholate(1 mg/kg) in the bile-pancreatic.Pancreatitis was evaluated by the histopathological score.Calculate lung wet/dry ratio.Serum amylase levels were measured by automatic biochemical analyzer.IL-6 and IL-10 levels were measured by enzyme-liked immunoadsordent assay(ELISA).P-ATF2 expression were determined by immunohistochemisry(IHC) staining.Results P-ATF2 expression level was a little bit low in SO group,P-ATF2 expression level at all related time points increased significartly in SAP group(P0.05);expression level at all time points in SB group was remarkable lower than that in SAP group(P0.05).IL-6 at 6 h and 12 h time points,IL-10 at 3 h and 6 h time points,serum amylase and ascites at all time points in SB group were lower than those in SAP(P0.05).Pancreatitic histopathological score at all time points in SB group were lower than those in SAP significantly(P0.05).Conclusion p38 MAPK signal transduction pathway could play a key role in the pathogenesis of SAP model.Therefore,p38 MAPK could represent a novel target for future therapies in SAP.