Component Analysis of Phenylethanoid Glycosides from Callicarpa kochiana Makino

Objective To study the high-polarity chemical constituents of Callicarpa kochiana Makino,thus to supply evidence for its pharmacological research and therapeutic mechanism.Methods The chemical constituents of Calli-carpa kochiana Makino were extracted by methanol and different polar solvents to obtain n-butanol extract.Then n-butanol extract was isolated and purified by macroporous resin,reversed phase silica gel,Sephadex LH-20 and Toyopearl HW-40.The structure of n-butanol extract was identified by spectral analysis of ultraviolet spectrum,in-frared spectrum,mass spectrum,and NMR spectrum.Results We obtained 8 chemical compounds from n-butanol extract.Of them,six phenylethanoid glycosides were identified as acteoside(1),forsythosides B(2),cistanoside C(3),isoacteoside(4),cistanoside D(5),lamiophlomioside A(6).Conclusion Compound 3,5 and 6 is isolated from Callicarpa genus plant for the first time,and phenylethanoid glycosides are the main chemical constituents from n-butanol fraction of Callicarpa kochiana Makino.