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2010•Zhongguo xin yao zazhiRequires access

Inhibitory effect of AL-017 on inducible nitric oxide synthase and cyclooxygenase-2

XU Guang-lin

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Abstract

Objective:To investigate the inhibitory effect of AL-017 on inducible nitric oxide synthase(iNOS) and cyclooxygenase-2(COX-2) and its possible molecular mechanism.Methods:Mouse macrophages(RAW264.7) were stimulated by lipopolysaccharide(LPS).NO release,total iNOS enzyme activity,mRNA expression levels of iNOS and COX-2,and p38 MAPK were analyzed after LPS stimulation.Results:AL-017(3μmol·L-1) decreased the LPS-induced NO production and increased iNOS activity with inhibitory rates of 77.2% and 70.0%,respectively.AL-017 also significantly reduced LPS-stimulated increases in iNOS and COX-2 mRNA,and p38 MAPK phosphorylation.Conclusion:AL-017 can prevent LPS-stimulated inflammation in RAW264.7 cells,which may be associated with the inhibition of p38 MAPK phosphorylation.

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Objective:To investigate the inhibitory effect of AL-017 on inducible nitric oxide synthase(iNOS) and cyclooxygenase-2(COX-2) and its possible molecular mechanism.Methods:Mouse macrophages(RAW264.7) were stimulated by lipopolysaccharide(LPS).NO release,total iNOS enzyme activity,mRNA expression levels of iNOS and COX-2,and p38 MAPK were analyzed after LPS stimulation.Results:AL-017(3μmol·L-1) decreased the LPS-induced NO production and increased iNOS activity with inhibitory rates of 77.2% and 70.0%,respectively.AL-017 also significantly reduced LPS-stimulated increases in iNOS and COX-2 mRNA,and p38 MAPK phosphorylation.Conclusion:AL-017 can prevent LPS-stimulated inflammation in RAW264.7 cells,which may be associated with the inhibition of p38 MAPK phosphorylation.

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Available abstract

Objective:To investigate the inhibitory effect of AL-017 on inducible nitric oxide synthase(iNOS) and cyclooxygenase-2(COX-2) and its possible molecular mechanism.Methods:Mouse macrophages(RAW264.7) were stimulated by lipopolysaccharide(LPS).NO release,total iNOS enzyme activity,mRNA expression levels of iNOS and COX-2,and p38 MAPK were analyzed after LPS stimulation.Results:AL-017(3μmol·L-1) decreased the LPS-induced NO production and increased iNOS activity with inhibitory rates of 77.2% and 70.0%,respectively.AL-017 also significantly reduced LPS-stimulated increases in iNOS and COX-2 mRNA,and p38 MAPK phosphorylation.Conclusion:AL-017 can prevent LPS-stimulated inflammation in RAW264.7 cells,which may be associated with the inhibition of p38 MAPK phosphorylation.

Key concepts: Nitric oxide synthase, Lipopolysaccharide, Cyclooxygenase, Nitric oxide, p38 mitogen-activated protein kinases, Chemistry, Stimulation, Phosphorylation

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