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SURVEY OF HCV INFECTION IN INTRAVENOUS INJECTION DRUG ABUSERS IN CHONGQING

Chengming Li

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Abstract

Objective:To investigate the prevalence of HCV infection in intravenous drug users (IDUs) and molecular biological characteristics of HCV. Methods: The serum samples from 284 IDUs were obtained, and anti-HCV IgG was detected by ELISA, HCV was typed by RT-PCR and restriction fragment length polymorphism ( RFLP ) analysis of the 5' untranslated region.Results: There were 115 cases (40.5%) anti-HCV IgG positive and 98 cases( 34.5%) HCV-RNA positive in the 284 IDUs. The HCV 1b and HCV 2a genotypes and the 1b/2a mixed genotype accounted for 33.7%, 46.9% and 19.4% of HCV infection, respectively. The distribution of HCV genotypes in the individuals with different levels of ALT was identical. Conclusion: Infection of HCV 2a genotype was predominant in the IDUs in Chongqing, followed by HCV 1b and 1b/2a mixed genotypes. The different genotypes of HCV were not related to the change of serum ALT levels.

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Objective:To investigate the prevalence of HCV infection in intravenous drug users (IDUs) and molecular biological characteristics of HCV. Methods: The serum samples from 284 IDUs were obtained, and anti-HCV IgG was detected by ELISA, HCV was typed by RT-PCR and restriction fragment length polymorphism ( RFLP ) analysis of the 5' untranslated region.Results: There were 115 cases (40.5%) anti-HCV IgG positive and 98 cases( 34.5%) HCV-RNA positive in the 284 IDUs. The HCV 1b and HCV 2a genotypes and the 1b/2a mixed genotype accounted for 33.7%, 46.9% and 19.4% of HCV infection, respectively. The distribution of HCV genotypes in the individuals with different levels of ALT was identical. Conclusion: Infection of HCV 2a genotype was predominant in the IDUs in Chongqing, followed by HCV 1b and 1b/2a mixed genotypes. The different genotypes of HCV were not related to the change of serum ALT levels.

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Available abstract

Objective:To investigate the prevalence of HCV infection in intravenous drug users (IDUs) and molecular biological characteristics of HCV. Methods: The serum samples from 284 IDUs were obtained, and anti-HCV IgG was detected by ELISA, HCV was typed by RT-PCR and restriction fragment length polymorphism ( RFLP ) analysis of the 5' untranslated region.Results: There were 115 cases (40.5%) anti-HCV IgG positive and 98 cases( 34.5%) HCV-RNA positive in the 284 IDUs. The HCV 1b and HCV 2a genotypes and the 1b/2a mixed genotype accounted for 33.7%, 46.9% and 19.4% of HCV infection, respectively. The distribution of HCV genotypes in the individuals with different levels of ALT was identical. Conclusion: Infection of HCV 2a genotype was predominant in the IDUs in Chongqing, followed by HCV 1b and 1b/2a mixed genotypes. The different genotypes of HCV were not related to the change of serum ALT levels.

Key concepts: Genotype, Virology, Medicine, Drug abuser, Restriction fragment length polymorphism, Intravenous drug, Hepatitis C virus, Immunology

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