Rapid Determination of Contents of Matrine and Oxymatrine in Radix Sophorae Tonkinensis by UPLC

Objective To study the rapid determination of oxymatrine(OM) and matrine(M) in Radix Sophorae Tonkinensis at the same time.Methods A UPLC method was established.The Waters ACQUITY uplcHSS T3 column was used.The mobile phase consisted of water-acetonitrile(with 0.1% triethylamine) with gradient elution at the flow rate of 0.5 ml/min,the detection wavelength was set at 220 nm.Results The linear range of oxymatrine was 20.16～201.6 μg/ml and matrine was 20.88～208.8 μg/ml,respectively.The average recoveries of oxymatrine and matrine were 99.1% with RSD=1.27%,and 99.5% with RSD=1.40%(n=9),respectively.Conclusion The method is rapid and accurate,and can be used for the quality control of Radix Sophorae Tonkinensis.The UPLC method is suitable for the rapid detection of Chinese herbal medicines.