Studies on Crucial Technology of Rapid Asexual Propagation and Commercial Production of Phalaenopsis

The major technology which affecting rapid asexual propagation and commercial production of Phalaenopsis was discussed.Results showed that the type of culture medium and the time of subculture directly affected the propagation rate of its protocorm.The propagation rates of protocorm in the modified Kyoto and KC media were significantly higher than that in MS medium.The optimal subculture time was 2.5 months.The maturity of seedlings was accelerated by using two steps cultivation,and it only needed seven or eight months in tube.According to investigation on the abloom plant of protocorm of five varieties propagated for 20 generations,the results showed that the colony characters of these blooming plants are stable and the rate of variation of two protocorms was lower than 1/10 000 in the propagation of 18th-20th generations.