Study on the preparation and purification of polyclonalantibody and enzyme labelled antibody of erythropoietin

Objective Purified the rhEPO using the immunoaffinity chromatography method,and immunized New Zealand rabbits to produce the EPO polyclonalantibody.Labeled the antibody with HRP,to prepare for establishing ELISA system.Methods Purchased the rhEPO,coupled the EPO antibody with sephadex to make the affinity column.Then,purify the rhEPO as the sample.With regard to the antibody,it should be primarily purified by the salting-out,and then purified with the SPA affinity column.Prepared the enzyme labelled antibody by the sodium metaperiodate method.Results The EPO antibody and antigen were highly purified,the protein contents were 0.56 and 0.41 mg/ml.the enzyme biding rate was 0.578.Conclusion Purified the rhEPO and enzyme labeled antibody using the immunoaffinity chromatography method and the they were highly purified,so it met the need of making the ELISA syystem.