Determination of Amino Acids in Nine Kinds of Coix Non Kernel Parts
Shao Jinmin
Abstract
Shao Jinmin
Abstract
In order to understand the amino acids content in Coix non kernel parts,and provide a reference for quality evaluation and selection and breeding of high-quality medicinal materials,the amino acids in non kernel parts of Coix,collected in Guizhou and Yunnan with different harvest time and origin, were determined by pre-column derivatization for RP-HPLC.The results showed that nine Coixnon kernel parts contained 16kinds of amino acids,which contained seven kinds of essential amino acids.Overall, within the Coixendopleura the total amino acids and essential amino acids were the highest,11 342~ 13 981mg/100g and 3 553~4 260mg/100g respectively,followed by Coixroots and testa,4 263~6 753 mg/100g and 1 373~1 683mg/100g in roots,1 537~6 851mg/100gand 506~1 751mg/100g in testa. Consequently,endopleura with short storage time could be comprehensively utilized in the exploitation of non kernel parts of Coix.
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In order to understand the amino acids content in Coix non kernel parts,and provide a reference for quality evaluation and selection and breeding of high-quality medicinal materials,the amino acids in non kernel parts of Coix,collected in Guizhou and Yunnan with different harvest time and origin, were determined by pre-column derivatization for RP-HPLC.The results showed that nine Coixnon kernel parts contained 16kinds of amino acids,which contained seven kinds of essential amino acids.Overall, within the Coixendopleura the total amino acids and essential amino acids were the highest,11 342~ 13 981mg/100g and 3 553~4 260mg/100g respectively,followed by Coixroots and testa,4 263~6 753 mg/100g and 1 373~1 683mg/100g in roots,1 537~6 851mg/100gand 506~1 751mg/100g in testa. Consequently,endopleura with short storage time could be comprehensively utilized in the exploitation of non kernel parts of Coix.
Key concepts: Coix, Amino acid, Chemistry, Derivatization, Kernel (algebra), High-performance liquid chromatography, Biology, Food science