The G6PD activity analysis of people carrying thalassemia genes

Objective: Try to understand the relation between glucose-6-phosphate dehydrogenase(G6PD) activity range and thalassemia,and investigate the meaning of G6PD inspection during diagnosis of thalassemia.Metheds: Blood examination screened high risk population of thalassemia,hemoglobin subtypes were detected by HPLC.α-thalassemia and β-thalassemia were tested by GAP-PCR and reverse dot blot(RDB).The G6PD activities of population carrying thalassemia gene were tested by rate method,divided normal group and increased group.Results: The G6PD activities of hemoglobin H disease,minor β,Standard α,Stationary α and non-deletion type α thalassemia all have various degrees of increase.This research didn't find out any severe patient with thalassemia being over 20-year-old.Conclusion: The increasing of G6PD plays a supporting role in the diagnosis of thalassemia.And the difference of the increasing degrees has reference value for the classifycation of thalassemia.