Effect of Proteasome Inhibitor Bortezomib on Proliferation,Apoptosis of Human Choriocarcinoma JEG3 Cells

Objective:To investigate the effect of proteasome inhibitor bortezomib on proliferation,apoptosis and the expression of NFκB of human choriocarcinoma JEG3 cells.Methods:JEG3 cells were treated with bortezomib at different concentrations(01～200 nmol/L).Cell proliferation was analyzed by CCK8 assay,cell apoptosis by flow cytometry and fluorescence microscope,NFκB protein expression by immunofluorescence,blank control was set.Results:JEG3 cells were treated with bortezomib at different concentrations for 48 h respectively,the cell proliferation inhibition rate gradually increased with the increase of drug concentration(P005).JEG3 cells was treated with bortezomib(10 nmol/L) for 24 h,48h and 72 h,cell proliferation inhibition rate gradually increased with the prolonging of drug action time (P001).Compared to the control group,JEG3 cells treated with 10 nmol/L bortezomib for 48 h showed nuclear condensation,nuclear fragmentation,apoptotic body formation and increase of the apoptotic cells rate(P001).The expression of NFκB in apoptotic cells after bortezomib treatment was significantly increased.Conclusions:Bortezomib can inhibit the proliferation of JEG3 cells,and induce apoptosis concentration and time dependently in a certain scope of concentration and reacting time,which maybe related to the increasing the NFκB expression.