Ginsenosides in Leaves of Panax notoginseng,P. ginseng and P. quinquefolius

Leaves of Panax notoginseng,P.ginseng and P.quinquefolius have similar saponin components but with different exclusive components.In this work,the contents of saponins in the leaves of Panax notoginseng,P. ginseng and P.quinquefolius were determined with reversed-phase high-performance liquid chromatography.The chromatographic separation was achieved by using a Kinetex-C18 column(100×4.6 mm,2.6 μm,phenomenex,at 30 ℃).Eleven compounds were separated with a gradient elution and detected at 203 nm.The results showed that the order of total content of saponins,protopanaxadiol saponins and protopanaxatriol saponins in three kinds of leaves was P.quinquefolius P.ginseng P.notoginseng,P.quinquefolius P.notoginseng P.ginseng,and P. ginseng P.quinquefolius P.notoginseng,respectively.The content of protopanaxadiol saponins in the leaves of P.quinquefolius and the content of protopanaxatriol saponins in the leaves of P.ginseng obviously were higher than those in others.And the total content of Ginsenosides Rb3 and Rd was accounted for over 60% of the total 11 ginsenosides in P.quinquefolius leaves.Conclusively,the leaves of Panax notoginseng,P.ginseng and P. quinquefolius should be well used as a wonderful source for saponins due to their high contents.In addition,all the three kinds of saponins should be exploited on the basis of their contents and compositions.