ScholarLens Back to search
2007•Zhongguo linchuang yaolixue yu zhiliaoxueRequires access

Effects of heparin on expression of leptin and transforming growth factor β_1 in rat hepatic stellate cells

Hongjiao Wang

Open publisher page 0 citations

Abstract

AIM:To explore the effects of heparin on the expression of leptin and transforming growth factor β1(TGF β1) in rat hepatic stellate cells(HSCs). METHODS: HSCs were divided as follows: heparin groupⅠ(10 μg/mL), Ⅱ(100 μg/mL), Ⅲ(1 000 μg/mL), and control group. The incubation liquid was extracted after cultured 48 h and frosted in -20 ℃. Leptin and TGF β1 were detected by ELISA, the HSC proliferation was measured by MTT assay. RESULTS: The levels of leptin in heparin groupⅡ,Ⅲ were significantly lower than that in control group(P0.01). The level of TGF β1 and the mean absorbance in heparin groups were significantly lower than that in control group(P0.01). CONCLUSION: Heparin can decrease expression of leptin and TGF β1 in HSCs and inhibit proliferation of HSCs.

About this research paper

What this paper is about

AIM:To explore the effects of heparin on the expression of leptin and transforming growth factor β1(TGF β1) in rat hepatic stellate cells(HSCs). METHODS: HSCs were divided as follows: heparin groupⅠ(10 μg/mL), Ⅱ(100 μg/mL), Ⅲ(1 000 μg/mL), and control group. The incubation liquid was extracted after cultured 48 h and frosted in -20 ℃. Leptin and TGF β1 were detected by ELISA, the HSC proliferation was measured by MTT assay. RESULTS: The levels of leptin in heparin groupⅡ,Ⅲ were significantly lower than that in control group(P0.01). The level of TGF β1 and the mean absorbance in heparin groups were significantly lower than that in control group(P0.01). CONCLUSION: Heparin can decrease expression of leptin and TGF β1 in HSCs and inhibit proliferation of HSCs.

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

AIM:To explore the effects of heparin on the expression of leptin and transforming growth factor β1(TGF β1) in rat hepatic stellate cells(HSCs). METHODS: HSCs were divided as follows: heparin groupⅠ(10 μg/mL), Ⅱ(100 μg/mL), Ⅲ(1 000 μg/mL), and control group. The incubation liquid was extracted after cultured 48 h and frosted in -20 ℃. Leptin and TGF β1 were detected by ELISA, the HSC proliferation was measured by MTT assay. RESULTS: The levels of leptin in heparin groupⅡ,Ⅲ were significantly lower than that in control group(P0.01). The level of TGF β1 and the mean absorbance in heparin groups were significantly lower than that in control group(P0.01). CONCLUSION: Heparin can decrease expression of leptin and TGF β1 in HSCs and inhibit proliferation of HSCs.

Key concepts: Leptin, Heparin, Hepatic stellate cell, Transforming growth factor, Endocrinology, Internal medicine, Chemistry, Growth factor

Related papers

Related paper 1Related paper 2Related paper 3
Back to paper searchBrowse research topicsOriginal source
Effects of heparin on expression of leptin and transforming growth factor β_1 in rat hepatic stellate cells — Research Paper | ScholarLens