The effect of siRNA-ΔNp63 on migration and invasion in HeLa cells in vitro

Objective To explore the effects of siRNA-ΔNp63 on migration and invasion in HeLa cells in vitro.Methods SiRNA-ΔNp63 was transfected into HeLa cells by lipidosom method in vitro.The cells were divided into control group(without transfection),empty vector group(only added lipo2000),negative plasmid group(transfected with negative siRNA) and inferference plasmid group(transfected with effective siRNA).The mRNA,protein expression level of ΔNp63 were assayed by real-time PCR and Western-blot.The invasion and migration of cell were detected by Transwell,homotypic and hetertypic cell adhesion experiment.Results The siRNA-ΔNp63 interference plasmid into HeLa cells was successful;compared with the control group,mRNA and protein expression of ΔNp63 in negative plasmid group and the empty vector group did not change significantly(P 0.05).mRNA and protein expression of ΔNp63 in interference plasmid group were decreased(P 0.05),the average cell penetrating number,homogeneous adhesion and heterogeneity adhesion in negative plasmid group and the empty vector group did not change(P 0.05),but decreased in the siRNA-ΔNp63 plasmid group about average cell penetrating number(P 0.05),homogeneous adhesion increase(P 0.05) and heterogeneity adhesion decreased(P 0.05).Conclusion The siRNA-ΔNp63 can decrease the function of migration and invasion in HeLa cells in vitro.