2010•Tianjin Yike Daxue xuebaoRequires access

Effect of ischemic postconditioning on apoptosis after focal cerebral ischemia reperfusion in rat

Wang Jizuo

Abstract

Objective:To investigate the effect of ischemic postconditioning on apoptosis after focal cerebral ischemia reperfusion in rat.Methods:A rat model of focal cerebral ischemia-reperfusion injury was established by middle cerebral artery occlusion using modified filament method.Male Sprague-Dawley rats were randomized into 3 groups(n=10):sham-operate(sham) group,ischemia/reperfusion(I/R) group and ischemic postconditioning(IP) group.TUNEL assay was employed to examine the cell apoptosis and Western blotting analysis were used to detect the expression of P38 and Caspase-3 proteins.Results:The apoptotic cells and protein expression of P38 and Caspase-3 increased markedly after cerebral ischemia-reperfusion injury in rats.While the apoptotic cells and protein expression of P38 and Caspase-3 decreased significantly in the IP group compared with the I/R group(P0.01).Conclusion:Ischemic postconditioning decrease the protein expression of P38 and Caspase-3 after focal cerebral ischemia reperfusion in rat,which might be an important mechanism of apoptosis.

About this research paper

What this paper is about

Why it matters

A significance statement is not available in the OpenAlex record.

Key contribution

A contribution statement is not available in the OpenAlex record.

Method / approach

Method details are not available in the OpenAlex metadata.

Main findings

Findings are not separately available in the OpenAlex metadata.

Limitations

Limitations are not available in the OpenAlex metadata.

Applications

Application details are not available in the OpenAlex metadata.

Available abstract

Key concepts: Ischemia, Apoptosis, TUNEL assay, Medicine, p38 mitogen-activated protein kinases, Reperfusion injury, Blot, Anesthesia