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Exploitation and Utilization of Wild Zhizi Resources(Sect.Phaeonema of Ganoderma)in Fujian(II).G.sinense“Minzi 96”

Chen Ti-qiang, Tcm Pharmacology

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Abstract

“Minzi 96”strain was successively domesticated from wild Zizhi resources(Sect.Phaeonema in Subgen.Ganoderma)in Fujian province,and was officially identified as Ganoderma sinense J.D.Zhao, L.W.Hsu et X.Q.Zhng(with exemplar number HAMS 77207),which accords with the statutory origin of Lingzhi(GANODERMA)medicinal material in the Chinese Pharmacopoeia(Ch.P).The strain was mas- sively apphed to log~cultivation with albumum in 2004 and 2005.Popularization scale was 860 m~(-3) (mycelia~colonized wood-logs,1m~3 equal to 1 100 kg),and average yield(in the primary year)reached 12.70 kg/m~3(dry fruitbodies).The fruitbodies were of moderate size,with 7.0~9.0×10.0~17.3 cm pileus,while most of the basdiospores were plumpy and intact,with a size of 6.84~7.37×10.26~11.05μm.The fruitbodies were processed into ultrafine-powder(throughΦ300 R40/3 sieve,GB/ T6003.2-1997)by cryogenic vibration mill at low temperature.The micro~morphology of ultrafine~powder was fiber~form,and the structure of context hyphae was disintegrated into fragments,with a length of 10~50μm,a diameter of 1.2~4.5μm,observed by scanning electron microscope(SEM).The granularity was tested by Laser grain~size analysis.The specific surface area(BET)was 227.54 m~2/kg, and the cumulative distribution data D97 was 63.8μm(equivalent grain-size).The analytic results showed that contents of moisture,crude protein,fat,crube fiber,total carbohydrate,ash and polysaccha- ride in the ultrafine-powder were 4.2 g/100g,10.84 g/100g,3.71 g/100g,36.5 g/100g,18.4 g/100g, 1.7g/100g and 1.3 g/100g respectively.Total content of seventeen kinds of amino acids was 9.30 mg/100mg,and the ratio of essential amino acids were 65.7%.The fatty acid composition includ- ed oleic acid(C18:1,45.5%),linoleic acid(C18:2,27.7%),palmitic acid(C16:0,18.8%)and others,nonsaturated fatty acids made up the majority.Moreover,contents of heavy metal elements Pb, As,Cd and Hg were less than 0.2μg/g,0.13μg/g,0.072μg/g and 0.24μg/g respectively,which co- incided with the hygienic standard of Green Trade Standards of Importing Exporting Medicinal Plants Preparations(WM2-2001).

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What this paper is about

“Minzi 96”strain was successively domesticated from wild Zizhi resources(Sect.Phaeonema in Subgen.Ganoderma)in Fujian province,and was officially identified as Ganoderma sinense J.D.Zhao, L.W.Hsu et X.Q.Zhng(with exemplar number HAMS 77207),which accords with the statutory origin of Lingzhi(GANODERMA)medicinal material in the Chinese Pharmacopoeia(Ch.P).The strain was mas- sively apphed to log~cultivation with albumum in 2004 and 2005.Popularization scale was 860 m~(-3) (mycelia~colonized wood-logs,1m~3 equal to 1 100 kg),and average yield(in the primary year)reached 12.70 kg/m~3(dry fruitbodies).The fruitbodies were of moderate size,with 7.0~9.0×10.0~17.3 cm pileus,while most of the basdiospores were plumpy and intact,with a size of 6.84~7.37×10.26~11.05μm.The fruitbodies were processed into ultrafine-powder(throughΦ300 R40/3 sieve,GB/ T6003.2-1997)by cryogenic vibration mill at low temperature.The micro~morphology of ultrafine~powder was fiber~form,and the structure of context hyphae was disintegrated into fragments,with a length of 10~50μm,a diameter of 1.2~4.5μm,observed by scanning electron microscope(SEM).The granularity was tested by Laser grain~size analysis.The specific surface area(BET)was 227.54 m~2/kg, and the cumulative distribution data D97 was 63.8μm(equivalent grain-size).The analytic results showed that contents of moisture,crude protein,fat,crube fiber,total carbohydrate,ash and polysaccha- ride in the ultrafine-powder were 4.2 g/100g,10.84 g/100g,3.71 g/100g,36.5 g/100g,18.4 g/100g, 1.7g/100g and 1.3 g/100g respectively.Total content of seventeen kinds of amino acids was 9.30 mg/100mg,and the ratio of essential amino acids were 65.7%.The fatty acid composition includ- ed oleic acid(C18:1,45.5%),linoleic acid(C18:2,27.7%),palmitic acid(C16:0,18.8%)and others,nonsaturated fatty acids made up the majority.Moreover,contents of heavy metal elements Pb, As,Cd and Hg were less than 0.2μg/g,0.13μg/g,0.072μg/g and 0.24μg/g respectively,which co- incided with the hygienic standard of Green Trade Standards of Importing Exporting Medicinal Plants Preparations(WM2-2001).

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Available abstract

“Minzi 96”strain was successively domesticated from wild Zizhi resources(Sect.Phaeonema in Subgen.Ganoderma)in Fujian province,and was officially identified as Ganoderma sinense J.D.Zhao, L.W.Hsu et X.Q.Zhng(with exemplar number HAMS 77207),which accords with the statutory origin of Lingzhi(GANODERMA)medicinal material in the Chinese Pharmacopoeia(Ch.P).The strain was mas- sively apphed to log~cultivation with albumum in 2004 and 2005.Popularization scale was 860 m~(-3) (mycelia~colonized wood-logs,1m~3 equal to 1 100 kg),and average yield(in the primary year)reached 12.70 kg/m~3(dry fruitbodies).The fruitbodies were of moderate size,with 7.0~9.0×10.0~17.3 cm pileus,while most of the basdiospores were plumpy and intact,with a size of 6.84~7.37×10.26~11.05μm.The fruitbodies were processed into ultrafine-powder(throughΦ300 R40/3 sieve,GB/ T6003.2-1997)by cryogenic vibration mill at low temperature.The micro~morphology of ultrafine~powder was fiber~form,and the structure of context hyphae was disintegrated into fragments,with a length of 10~50μm,a diameter of 1.2~4.5μm,observed by scanning electron microscope(SEM).The granularity was tested by Laser grain~size analysis.The specific surface area(BET)was 227.54 m~2/kg, and the cumulative distribution data D97 was 63.8μm(equivalent grain-size).The analytic results showed that contents of moisture,crude protein,fat,crube fiber,total carbohydrate,ash and polysaccha- ride in the ultrafine-powder were 4.2 g/100g,10.84 g/100g,3.71 g/100g,36.5 g/100g,18.4 g/100g, 1.7g/100g and 1.3 g/100g respectively.Total content of seventeen kinds of amino acids was 9.30 mg/100mg,and the ratio of essential amino acids were 65.7%.The fatty acid composition includ- ed oleic acid(C18:1,45.5%),linoleic acid(C18:2,27.7%),palmitic acid(C16:0,18.8%)and others,nonsaturated fatty acids made up the majority.Moreover,contents of heavy metal elements Pb, As,Cd and Hg were less than 0.2μg/g,0.13μg/g,0.072μg/g and 0.24μg/g respectively,which co- incided with the hygienic standard of Green Trade Standards of Importing Exporting Medicinal Plants Preparations(WM2-2001).

Key concepts: Context (archaeology), Ganoderma, Mycelium, Hypha, Botany, Horticulture, Biology, Food science

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